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cd25 2a3 fluidigm 3169003b surface ab 2938861 170 er tcr va24 ja18 (fluidigm)

Name: cd25 2a3 fluidigm 3169003b surface ab 2938861 170 er tcr va24 ja18
Brand: fluidigm
Rating: 94 (40 reviews)

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fluidigm cd25 2a3 fluidigm 3169003b surface ab 2938861 170 er tcr va24 ja18
Cd25 2a3 Fluidigm 3169003b Surface Ab 2938861 170 Er Tcr Va24 Ja18, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd25 2a3 fluidigm 3169003b surface ab 2938861 170 er tcr va24 ja18/product/fluidigm
Average 94 stars, based on 40 article reviews

cd25 2a3 fluidigm 3169003b surface ab 2938861 170 er tcr va24 ja18 - by Bioz Stars, 2026-03

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a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and <t>CD19−NK1.1−CD11b−CD11c−TCRβ+</t> (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.

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a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and CD19−NK1.1−CD11b−CD11c−TCRβ+ (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.

Journal: Nature

Article Title: Clonally expanded CD8 T cells characterize Amyotrophic Lateral Sclerosis 4

doi: 10.1038/s41586-022-04844-5

Figure Lengend Snippet: a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and CD19−NK1.1−CD11b−CD11c−TCRβ+ (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.

Article Snippet: The following isotope conjugated anti-mouse antibodies were used at 1:100 dilution: 113 In CD45.1 (clone A20, eBioscience, Catalog # 14–0453-85), 115 In CD45.2 (clone 104, eBioscience, Catalog # 14–0454-85), 141 Pr Ly6G (clone 1A8, Fluidigm, Catalog # 3141008B), 142 Nd CD11c (clone N418, Biolegend, Catalog # 117302), 143 Nd TCRbeta (clone H57–597, Fluidigm, Catalog # 3143010B), 144 Nd CD24 (clone M1/69, Biolegend, Catalog # 101802),145 Nd CD69 (clone H1.2F3, Fluidigm, Catalog # 3145005B), 146 Nd F4/80 (clone BM8, Fluidigm), 147 Sm CD357/GITR (clone DTA-1, Biolegend), 148 Nd CD11b (clone M1/70, Biolegend, Catalog #), 149 Sm CD19 (clone 6D5, Fluidigm, Catalog #), 150 Nd IgD (clone 11–26c.2a, Biolegend, Catalog #), 151 Eu CD25 (clone 3C7, Fluidigm, Catalog # 3146008B), 152 Sm SiglecF (clone S17007L, Biolegend, Catalog # 155502), 153 Eu NKp46 (clone 29A1.4, Fluidigm, Catalog # 3153006B), 155 Gd CD73 (clone TY/11.8, Biolegend, Catalog # 127202), 156 Gd CD64 (clone X54–5/7.1, Biolegend, Catalog # 139302), 158 Gd CD117 (clone 2B8, Biolegend, Catalog # 105802), 159 Tb PD-1 (clone RMP1, Biolegend, Catalog #), 160 Gd CD62L (clone MEL-14, Fluidigm, Catalog # 109113),162 Dy Ly6C (clone HK1.4, Biolegend, Catalog # 128002),163 Dy anti-APC (Biolegend, Catalog # 408002),164 Dy Sca-1 (clone D7, Fluidigm, Catalog # 3164005B), 165 Ho anti-PE (Biolegend, Catalog # 408102), 166 Er KLRG1 (clone 2F1/KLRG1, Biolegend, Catalog # 93135),167 Er CXCR3 (clone CXCR3–173, Biolegend, Catalog # 126526), 168 Er CD8alpha (clone 53–6.7, Biolegend, Catalog # 100702), 169 Tm CD206 (clone C068C2, Fluidigm, Catalog # 3169021B), 170 Er NK1.1 (clone PK136, Fluidigm, Catalog # 3170002B), 171 Yb CD44 (clone IM7, Biolegend, Catalog # 103002), 172 Yb CD4 (clone RM4–5, Fluidigm, Catalog # 3172003B), 174 Yb MHCII (I-A/I-E) (clone M5/114.15.2, Biolegend, Catalog # 107602), 175 Lu CD127 (clone A7R34, Biolegend, Catalog # 135002), 176 Yb B220 (RA3–6B2, clone Biolegend, Catalog # 103202).

Techniques: Mass Cytometry, Expressing, Flow Cytometry

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood

doi: 10.1007/978-1-0716-2553-8_10

Figure Lengend Snippet: Whole blood phosphoflow panel 1

Article Snippet: 142 Nd cCasp3 D3E9 Fluidigm 3142004A 143 Nd CD19 HIB19 Biolegend 302202 144 Nd pPLCg2 [Y759] K86-689.37 Fluidigm 3144015A 145 Nd CD4 RPA-T4 Fluidigm 3145001B 146 Nd IgD IA6-2 Fluidigm 3146005B 147 Sm CD20 2H7 Fluidigm 3147001B 148 Nd IgA Polyclonal Fluidigm 3148007B 149 Sm CD25 2A3 Fluidigm 3149010B 150 Nd pStat5 [Y694] 47 Fluidigm 3150005A 151 Eu CD123 6H6 Fluidigm 3151001B 153 Eu pStat1 [Y701] 4a Fluidigm 3153005A 154 Sm CD45 HI30 Fluidigm 3154001B 155Gd CD27 L128 Fluidigm 3155001B 156 Gd p38 [T180/Y182] D3F9 Fluidigm 3156002A 157 Gd CD24 ML-5 Biolegend 311102 158 Gd pStat3 [Y705] 4/P-Stat3 Fluidigm 3158005A 159Tb CD11c Bu15 Fluidigm 3159001B 160Gd CD14 M5E2 Fluidigm 3160001B 161 Dy CD141(BDCA-3) AD5-14H12 Miltenyi 130-090-694 162 Dy CD66b 80H3 Fluidigm 3162023B 163 Dy CD56 NCAM16.2 Fluidigm 3163007B 164 Dy IkBa L35A5 Fluidigm 3164004A 165 Ho pCREB [S133] 87G3 Fluidigm 3165009A 166 Er CD16 B73.1 Biolegend 360702 167 Er CD38 HIT2 Fluidigm 3167001B 168 Er CD8 SK1 Fluidigm 3168002B 169 Tm CD45RA HI100 Fluidigm 3169008B 170 Er CD3 UCHT1 Fluidigm 3170001B 171 Yb pERK1/2 [T202/Y204] D13.14.4E Fluidigm 3171010A 172 Yb Anti-Ki-67 B56 Fluidigm 3172024B 174 Yb HLA-DR L243 Fluidigm 3174001B 175Lu CD7 CD7-6B7 Biolegend 343102 176 Yb CD127/IL-7Ra P48-48 Novus Bio MAB306-100 209Bi CD11b/Mac-1 ICRF44 Fluidigm 3209003B Open in a separate window 1 Open channels are not shown but include Pd channels, Cd channel, Pt channels, 89Y,152Sm and 173Yb Whole blood phosphoflow panel1.

Techniques: